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Tuesday, April 21, 2020 | History

3 edition of Tracer kinetic studies of glucose and lactate metabolism in trained and untrained animals found in the catalog.

Tracer kinetic studies of glucose and lactate metabolism in trained and untrained animals

Tracer kinetic studies of glucose and lactate metabolism in trained and untrained animals

  • 279 Want to read
  • 7 Currently reading

Published .
Written in English

    Subjects:
  • Energy metabolism,
  • Blood -- Analysis,
  • Exercise -- Physiological aspects

  • Edition Notes

    Statementby Casey Michael Donovan
    The Physical Object
    FormatMicroform
    Paginationxi, 108 leaves
    Number of Pages108
    ID Numbers
    Open LibraryOL14640226M

      The liver converts fructose into other useful substances, such as carbohydrates (glucose or lactate) or fat, which are then delivered to various parts of the body via the bloodstream, to be used as fuel. So net net, a combination of both glucose and fructose is optimal for maximizing your absorption of calories. A male patient has had a successive fasting plasma glucose readings of , , and mg/dl. The healthcare provider will inform him that he appears to have impairment or borderline impairment of insulin-mediated glucose uptake by his cells. Student s test was used to compare the animals lactate or glucose concentrations before (T 0) and after 90 days of training, at the same exercise intensity (P. Production of fuels and chemicals through microbial fermentation of plant material is a desirable alternative to petrochemical-based production. Fermentative production of biorenewable fuels and chemicals requires the engineering of biocatalysts that can quickly and efficiently convert sugars to target products at a cost that is competitive with existing petrochemical-based by:


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Tracer kinetic studies of glucose and lactate metabolism in trained and untrained animals Download PDF EPUB FB2

Get this from a library. Tracer kinetic studies of glucose and lactate metabolism in trained and untrained animals. [Casey Michael Donovan]. Examples of Published Glucose Kinetic Studies with Stable Isotope Analysis Performed by Metabolic Solutions. Teff KL, Rickels MR, Grudziak J, Fuller C, Nguyen HL, and Rickels K.

Antipsychotic-induced insulin resistance and postprandial hormonal dysregulation independent of weight gain or psychiartric disease. Keywords. myocardial lactate oxidation; l C-lactate; PET; kinetic modeling; Derangements in myocardial carbohydrate metabolism have been associated with impairment in cardiac function in diverse conditions ranging from normal aging (1,2) to diabetes mellitus (3,4), ischemic heart disease (), and heart failure ().Because of its inherent quantitative capabilities.

Introduction. We are developing 18 F-labeled 6-fluorodeoxy-D-glucose ([18 F]6FDG) as a tracer of glucose transport. As part of this process it is important to characterize and quantify putative metabolites. In contrast to the ubiquitous PET tracer 18 F-labeled 2-fluorodeoxy-D-glucose ([18 F]2FDG) which is phosphorylated and trapped intracellularly, the substitution of Cited by: 4.

Lactate in the brain: from metabolic end-product to signalling molecule D. et al. Glucose and lactate metabolism in the awake and G. Postmortem studies in mood disorders indicate altered Cited by: Abstract.

Results of kinetic tracer studies on several mammalian species during rest and prolonged sustained exercise indicate that lactate is a metabolic intermediate which is very active in supplying carbon for a number of important physiological by: 3.

The results showed significant differences in the mobilization and utilization of muscle fuels between the athletically fit cyclists and the untrained group.

In the cyclists, glucose, glycerol and free fatty acid concentrations were higher, but lactate, pyruvate and alanine were lower than in the untrained subjects during exercise. by: Glucose solutions. To quantify exogenous glucose oxidation, solutions were prepared from corn-derived glucose (Amylum), which has a high natural abundance of 13 C.

The 13 C enrichment of the glucose was − δ/mil (‰) vs. Pee Dee Bellemnitella (PDB; − atom percent excess) and was determined by on-line combustion-isotope ratio mass spectrometer (Carlo Erba Cited by: Glucose is the major energetic substrate for the brain but evidence has accumulated during the last 20 years that lactate produced by astrocytes could be an additional substrate for neurons.

Aerobic metabolism of glucose from glycogen is advantageous over anaerobic metabolism of glucose from glycogen as it provides approximately lactate, glucose After an endurance training program, at a given relative workload (e.g. 65% of pretraining VO2max vs 65% of post-training VO2max) plasma _______ levels will be reduced compared to before.

In the past few years, the number of applications of tracers for in vivo biomedical studies has greatly increased. New analytical tools at the genetic and protein levels have spurred this growth, opening the door for a deeper understanding of metabolic events. This in turn promises to yield significant advances in the understanding and treatment of human disease.

Although several studies have assessed glucose kinetics during 1–2 h of strenuous exercise when trained subjects were fed carbohydrate (14, 15, 18), we are aware of only one case study in which glucose kinetics during exercise to the point of fatigue has been examined when subjects were fed carbohydrate.

Thus our first aim was to undertake Cited by:   Blood lactate production and recovery from anaerobic exercise were investigated in 19 trained (AG) and 6 untrained (CG) prepubescent boys.

The exercises comprised 3 maximal test performances; 2 bicycle ergometer tests of different durations (15 s and 60 s), and running on a treadmill for ± min to measure maximal oxygen uptake. Blood samples were taken Cited by: Glucose is the major energetic substrate for the brain but evidence has accumulated during the last 20 years that lactate produced by astrocytes could be an additional substrate for neurons.

However, little information exists about this lactate shuttle in vivo in activated and awake animals. We designed an experiment in which the cortical barrel field (S1BF) was unilaterally activated Cited by: Results of kinetic tracer studies on several mammalian species during rest and prolonged sustained exercise indicate that lactate is a metabolic intermediate which is Author: George A Brooks.

glucose and lactate metabolic protein expression in skeletal muscle. We have evaluated interventions that improve glucose and lactate metabolism in blood and muscles of diabetic animals including diet, physical exercise, and the use of pharmaceutical and nutriceutical compounds.

Recently, epidemiological studies. Introduction. We are developing 18 F-labeled 6-fluorodeoxy-d-glucose ([18 F]6FDG) as a tracer of glucose transport. As part of this process it is important to characterize and quantify putative metabolites.

In contrast to the ubiquitous positron emission tomography (PET) tracer 18 F-labeled 2-fluorodeoxy-d-glucose ([18 F]2FDG) which is phosphorylated and trapped intracellularly, Cited by: 4. Hormonal and Metabolic Responses of Untrained, Moderately Trained, and Highly Trained Men to Three Exercise Intensities P.A.

Deuster, G.P. Chrousos, A. Luger, J.E. DeBolt, L.L. Bernier, U.H. Trostmann, S.B. Kyle, L.C. Montgomery, and D.L. Loriaux Untrained, moderately trained (runners, 15 to 25 mi/wk), and highly trained (runners, >45 mi/wk) men participated in graded treadmill Cited by: Conclusions and Clinical Relevance—Results indicated that a single IET resulted in transient increases in MCT1 and MCT4 mRNA expression and protein content in untrained and trained horses.

These results may be important for the elucidation of exercise-induced alterations in lactate by: - converts lactate to glucose in liver. untrained subjects blood lactate levels begin to rise around __ to ___% VO2 max.

50/ trained subjects blood lactate levels rise around ___ to ___ % VO2 max. 65/ components of planning training programs around lactate threshold.

The goal of this book is to provide the researcher a solid theoretical base in tracer kinetics as applied to physiological metabolism. The book is a revised edition of “Radioactive and Stable Isotope Tracers in Biomedicine: Principles and Practice of Kinetic Analysis”.Cited by: This book covers the principal reports and discussions which have been presented during the "Symposium on Anaerobic Metabolism and Lactic Acid" which was held in Anzere (Wallis, Switzerland) from 19th till 20th January under the patronage of the European Society of Cardiology.

~s Dr. Wilkie and Hermansen mentioned in their paper, Berzelius was the first one. Techniques will be presented for investigating whole body metabolism, for metabolite balance across organs, intracellular flux rates and pathway regulation. General aspects of modeling will be considered, as well as specific applications in the study of carbohydrate, fat, protein metabolism and energy balance.

isotope tracers in metabolic research principles and practice of kinetic analysis course outline april 18 – 22, faculty henri brunengraber course co-director shawn c.

burgess gary cline nicolaas e. deutz joanne kelleher craig malloy owen mcguinness bettina mittendorfer stephen previs michelle puchowicz robert r.

wolfe course director general information. Muscle glycogen, which was also higher in trained than in untrained rats, was resynthesized at approximately the same rate in the two groups of animals (9 and 11 mumol/(g h), but the trained animals were able to achieve this without further depletion of liver glycogen beyond that which had occurred during by: Abstract.

A new two-pool model to describe glucose kinetics in the steady state is presented. The pools are plasma glucose, Q 1, and tissue glucose, Q 2 (both µmol).

The flows (all µmol/min) into the plasma pool (Pool 1) are absorbed glucose entry from dietary sources, labelled glucose infusion, and hepatic glucose is one flow out of Pool 1, glucose uptake by Author: Leslie L.

McKnight, Anna K. Shoveller, Secundino Lopez. control group at 27 min rose over % and in the trained group at 51 min, %. The lactate concentration at exhaustion in the trained group was significantly higher (an increase of %; P.

Five previously untrained yearling sled dogs were evaluated for endurance training-induced changes in maximum oxygen consumption (VO 2max) and submaximal blood lactate concentrations.

Following 3 weeks of light training followed by 4 weeks of moderate training, VO 2max increased by 10%, from ± to ± ml kg min − 1 (P = 0 Cited by: 6. programmed to read blood glucose lower than it actually is, at the low end of the scale (to prevent missing WsaVaFasaVa World congress Proceedings canIne dIaBeTes MellITUs Reprinted in IVIS with the permission of WSAVA Close this window to return to IVIS 36th World Small Animal Veterinary Congress - Jeju, Korea.

The Resource offers analysis of stable isotope enrichment of glucose, glycerol, fatty acids and amino acids in samples from metabolic tracer studies. These data can then be used to calculate rates of turnover, synthesis, production or recycling.

The Resource is currently located in room A Translational Research Center (TRC). In both fed and fasted mice, 13C-lactate extensively labels TCA cycle intermediates in all tissues. Quantitative analysis reveals that during the fasted state, the contribution of glucose to tissue TCA metabolism is primarily indirect (via circulating lactate) in all tissues except the l: Nature.

Isotope Tracers in Metabolic Research: Principles and Practice of Kinetic Analysis by Robert R. Wolfe () Hardcover – January 1, out of 5 stars 4 ratings See all 4 formats and editions Hide other formats and editions/5(4). "Isotope Tracers in Metabolic Research, Second Edition" offers students and researchers a comprehensive, practical resource for utilizing the latest tracer methodologies.

From the Back Cover In the past few years, the number of applications of tracers for in vivo biomedical studies has greatly increased/5(4). The PRKAG3 mutation carriers had higher glycogen storage, and resynthesis of glycogen was faster after 3 h but not after 6 h of recovery.

Alterations in the concentrations of insulin, glucose, lactate and free fatty acids after exercise did not differ between the genotypes. Both exercise groups performed the same amount of work and trained 5 days/wk for 7 wk.

To evaluate muscle insulin resistance, rat hindlimbs were perfused for 30 min with perfusate containing 6 mM glucose ( mu Ci of D-[14C(U)] glucose/ml) and either a maximal ( mU/ml) or a submaximal ( mU/ml) insulin concentration.

This study investigated fiber type-specific metabolic responses and the molecular mechanisms that regulate glucose and fat metabolism in oxidative and glycolytic muscles upon cold by: 6.

Several studies in horses have employed an infusion of a stable isotopically labeled tracer of glucose in order to calculate rates of glucose production (mainly hepatic glucose production) and utilization (mainly muscle glucose uptake) in horses during submaximal exercise.

63–69 In contrast with measurement of blood glucose concentrations. At this time point insulin-stimulated glucose uptake was 70 [plusmn] 21% higher (p[lt]) in the trained compared to the untrained leg as determined by a two hour euglycemic-hyperinsulinemic clamp procedure. Protein content of IR, IRS-1, P85, GSK-3[alpha], GSK-3[beta], Akt(1+2), PKC[zeta], GS and GLUT4 was determined by western blotting in.

Introduction. More than a century ago, increased glucose uptake was observed in the equine masseter muscle during chewing (Chauveau & Kaufmann, ).Studies in the s confirmed that contractions increased muscle glucose uptake in rats and dogs (Goldstein et al. ; Huycke, ) and in the s and s measurements of muscle glucose uptake Cited by: 8.

It has been suggested that the adipokine resistin links obesity and insulin resistance, although how resistin acts on muscle metabolism is controversial. We aimed to quantitatively analyse the effects of resistin on the glucose metabolic flux profile and on insulin response in L6E9 myotubes at the metabolic level using a tracer-based metabolomic approach Cited by: 7.

3 The Role of Glucose and FDG Metabolism in the Interpretation of PET Studies Ronald L. Korn, Alison Coates, and John Millstine. Ask most physicians to recall the enzymatic pathways of glucose metabolism, and you will likely provoke memories of premedical or medical school biochemistry classes where the common dictum was, “Glucose metabolism?Nearly 50 years ago, I set out to investigate the clinical problem of hypoglycemia in children with illnesses that limited their food intake.

My goal was to gather accurate and precise measurable data. At the time, I wasn't interested in nutrition as a discipline defined in its more general or popular sense.

To address the specific problem that interested me required development of Cited by: 2.esponse of R lood Lactate B, Glucose, Peripheral Oxygen Saturation, and Heart Rate during the rail T Running Competition.

JEP. online. ;19(2) This study evaluated the response of blood lactate, HR, SpO. 2, and blood glucose levels during a competition in different pathways (5km and 21km) and different levels of difficulty.

Th study.