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4 edition of Development and Validation of a Localized Murine Candidiasis Model found in the catalog.

Development and Validation of a Localized Murine Candidiasis Model

Faisal Abdi Guhad

Development and Validation of a Localized Murine Candidiasis Model

The Pathogenesis, Chemotherapy and Defense Mechanisms to Candida Mastitis in the Lactating ... Summaries of Uppsala Dissertations, 829)

by Faisal Abdi Guhad

  • 391 Want to read
  • 2 Currently reading

Published by Uppsala Universitet .
Written in English

    Subjects:
  • Alternative Medicine,
  • Medical

  • The Physical Object
    FormatPaperback
    ID Numbers
    Open LibraryOL12854049M
    ISBN 109155444288
    ISBN 109789155444280

    Patterson, T. Effect of antifungal treatment in a diet-based murine model of disseminated candidiasis acquired via the gastrointestinal tract Antimicrob Agents Chemother Jan; Vautier S, Drummond RA, Chen K, Murray GI, Kadosh D, Brown AJ, Gow NA, MacCallum DM, Kolls JK, Brown GD. Candida albicans is a well-known example of a pleomorphic fungus[1]. Pleomorphic micro-organisms can change form such that fungi might be a mould in some conditions and a yeast in other conditions. Thrush, can be identified as a mould and yeast can be diagnosed as Candidiasis, but .


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Development and Validation of a Localized Murine Candidiasis Model by Faisal Abdi Guhad Download PDF EPUB FB2

A murine model of localized candidiasis (mastitis) was developed. The model was analyzed withrespect to its discriminative abilities through investigation of the virulence properties of ns mutant strains compared with the wild-type parental strain. Further validation of themodel was undertaken by assessment of pathogenesis, chemotherapy (amphotericin B andfluconazole) and complement activation using immunocompetent BALB/c mice, SCID andathymic nude mice.

Development and validation of a localized murine candidiasis model. PhD thesis. Development and validation of a localized murine candidiasis model.

PhD thesis. Guhad, FA. Uppsala University, Department of Comparative Medicine. (Hau) (English) Book (Other scientific) Place, publisher, year, edition, pages. Development and validation of a new localized candidosis model: the Murine Mycotic mastitis Model Guhad, FA Uppsala University, Department of Comparative Medicine.

This murine model is performed using immunocompromised mice. Immunosuppression is achieved by: • Administration of cyclophosphamide (Cytoxan NDC# ) at mg/kg intraperitoneally (IP) and prednisolone sodium succinate (Solu-Delta-Cortef NADA# ) at 50 mg/kg subcutaneously (SC) on Days 14 and 17 post- inoculation.

Candida albicans. Murine Gastrointestinal Model for Disseminated Candidiasis (antibiotic approach) NIH/NIAID Task Order A13 Isolates: The murine model of gastrointestinal candidiasis described below has been validated with two separate wild-type isolates of.

Candida albicans. that are susceptible to clinically available antifungal agents. Using a murine model of systemic candidiasis, we conducted single-dose dose-ranging studies with fluconazole to determine the dosage of this drug that resulted in a 50% reduction in fungal densities (50% effective dose [ED 50 ]) in kidneys versus the fungal densities in the kidneys of untreated by: A Novel Murine Model of Oral Candidiasis with Local Symptoms Characteristic of Oral Thrush Article in Microbiology and Immunology 47(5) February with Reads How we measure 'reads'.

NIH/NIAID Small Animal Model Development and Utilization for Contract No. HHSNI Target Identification and Identfication and Testing of Task Order A13 - HHSN (Version ) Diagnostics, Therapeutics and Vaccines for Fungal Diseases Standard Operating Procedure (SOP) Candida albicans Murine Invasive Candidiasis Model.

The most widely used model for disseminated candidiasis is the murine intravenous model. Following injection of Candida cells in the tail vein, the survival rate. Murine models of oropharyngeal candidiasis have been used for investigating the immunology of this infection 11–14, candidal virulence factors 15–23, and the efficacy of vaccines and antifungal agents 24– Multiple experimental models of oropharyngeal candidiasis have been developed (reviewed in 27).

The key difference among these models is the type of immunosuppression that is used to induce susceptibility to oropharyngeal candidiasis. Publisher Summary. This chapter reviews the technical aspects of the experimental models of C.

albicans and C. glabrata vaginal infections in mice. Female mice is employed in the experimental model of C. albicans vaginal infection whose age should be greater than 6 weeks of age and weight is greater than 20 by: 1.

Introduction. Candida albicans (C. albicans), one of the most relevant fungal pathogens, causes different types of superficial infections including oral and vaginal candidiasis, and can disseminate systemically leading to high rate of morbidity and ling evidence shows that the oral cavity can be the site of origin for dissemination of pathogenic microorganisms to distant Cited by: 9.

No sign of systemic spread was observed in all of the infected animals when a postmortem examination was Development and Validation of a Localized Murine Candidiasis Model book. Therefore, this approach constituted a relevant model of naturally occurring fungal infection of a single organ. In the present study, we used the localized murine mastitis model to investigate the virulence of a C.

albicans strain with a deletion of the CEK1 MAP kinase gene and the Cited by: Read Online Development and Validation of a Localized Murine Candidiasis Model: The Pathogenesis, Chem EBOOK Read Online Performance Measurement and Leisure Management From Brand: Routledge EBOOK Read Online Soaps: The Spa Collection (Little Books) By Andrews McMeel Publishing; Smallwood EBOOK.

UME6 expression promotes tissue invasion in a reconstituted human model of oropharyngeal candidiasis. In order to determine the effect of C. albicans UME6 expression on biofilm formation and.

model of disseminated candidiasis caused by C. albicans Kq2 4h,q 1 2h,q6h,a n dq3h, dosing ev 12, 6, and 3 h, respectively; the values in parentheses represent 95% con fi dence. Read Online Development and Validation of a Localized Murine Candidiasis Model: The Pathogenesis, Chem EBOOK Read Online Tapering and Peaking for Optimal Performance By Iñigo Mujika EBOOK Read Online The historical background of angina pectoris By Paul Dudley White EBOOK.

The most common murine model of candidiasis entails injection of conidial yeast forms of Candida into the tail vein (18). Dissemination occurs to all major organs, predominantly liver, and is.

We exploited the advantages of this new model of mucosal candidiasis to demonstrate localized NF-κB activation by C. albicans in vivo, finding that it can occur at all infection levels.

This is consistent with in vitro evidence that NF-κB is activated in epithelial cells by both high and low levels of C. albicans infection ( Cited by: For the murine vaginal candidiasis model, female CBA/J mice, 8 to 10 weeks old and weighing ∼20 g, were obtained from the National Institutes of Health (National Cancer Institute [NCI], Frederick, MD).Cited by: Publisher Summary.

This chapter presents a brief history of the development of animal models of infection. In the last three decades of the 19th century, experimental pathologists tried to further their knowledge of "spontaneous diseases", by comparing "induced pathological phenomena" with "natural morbid phenomena.".

To investigate the expression of vaginal IL and its role in experimental murine vaginal candidiasis and its relationship with infection and immune status, immuno-competent (group A) and immuno-suppressed (group B) murine models of vaginal candidiasis were established in estrogen-treated mice.

Non-estrogen-treated mice were used as controls (group C).Cited by: 2. This model revealed that all of the rod and housekeeping genes were predicted to be localized to the euchromatin domain in adult retina.

However, 14% (32/) of retinal progenitor genes and 53% (/) of non-rod cell-type-specific genes (e.g., Grik2, Gria3, Ctn4, Epha5, Cav1) were predicted to be localized to the heterochromatin domain Cited by: 3. Oral candidiasis, commonly referred to as “thrush,” is an opportunistic fungal infection that commonly affects the oral mucosa.

The main causative agent, Candida albicans, is a highly versatile commensal organism that is well adapted to its human host; however, changes in the host microenvironment can promote the transition from one of commensalism to : Taissa Vila, Ahmed S. Sultan, Daniel Montelongo-Jauregui, Mary Ann Jabra-Rizk.

Murine model. A standardized murine model was performed based on the model described by Smith et al. (19). Male 6- to 7-week-old ICR (CD1 specific-pathogen-free) mice (Criffa, Barcelona, Spain) weighing about 30 g each at the time of inoculation were used in Cited by: BOOK SELECTION SECTIONS ON THIS PAGE: BOOKS WITH GENERAL CANDIDA INFORMATION BOOKS WITH DETAILED & SCIENTIFIC CANDIDA INFORMATION CANDIDA BOOKS OF HISTORICAL INTEREST.

The following are some of the bestsellers on the topic of Candidiasis, along with a couple of more technical books for those interested in scientific details. Murine Candida albicans GI Candidiasis (dietary approach) SOP 4.

References: 1. Rahman D, Mistry M, Thavaraj S, et al. Murine model of concurrent oral and vaginal Candida albicans colonization to study epithelial host-pathogen interactions. Microbes and Infection ; 2. Candida albicans is an opportunistic fungal pathogen that causes superficial and systemic infections in humans (5).

Infection arises when the yeast is able to overcome the host immune response, and this interplay is regulated by pro- and anti-inflammatory by: Device-associated microbial growth, including Candida biofilms, represents more than half of all human microbial infections and, despite a relatively small risk of implant-associated diseases, this type of infection usually leads to high morbidity, increased health-care costs and prolonged antimicrobial therapy.

Animal models are needed to elucidate the complex host–pathogen interactions Cited by:   Developing animal models for polymicrobial diseases.

A murine model has been developed to reproduce the pathogenesis of human meningococcaemia, localized infections with Candida Cited by:   The superiority of rAls3p-N was seen in both a steroid-treated model of oropharyngeal candidiasis and an immunocompetent model of candidal vaginitis.

We have recently reported that our murine model of hematogenously disseminated candidiasis recapitulates the most severe form of disseminated candidiasis in humans, candidal septic by:   Development and prospective validation of a model estimating risk of readmission in cancer patients.

Carl R. Schmidt MD; Jennifer Hefner PhD, MPH; Ann S. McAlearney ScD, MS; Lisa Graham MSN; Kristen Johnson MHA; Susan Moffatt‐Bruce MD, PhD; Timothy Huerta PhD, MS; Timothy M. Pawlik MD, MPH, PhD; Susan White PhD; Pages: ; First.

Csank, C. et al. Roles of the Candida albicans mitogen-activated protein kinase homolog, Cek1p, in hyphal development and systemic candidiasis. Infect.

Immun. 66, – ()Cited by:   Here we use the transparent zebrafish swimbladder to model mucosal candidiasis. We show that this infection reproduces important aspects of fungal-epithelial interaction previously characterized in vitro, in murine models and in human find that high-level infection induces strong activation of NF-κB, transcriptional upregulation of NF-κB-dependent proinflammatory gene Cited by: Which Candida Virulence Factors Influence the Outcome of Invasive Candidiasis?.

Candida expresses a variety of virulence factors that contribute to its pathogenesis and could be exploited for development of vaccines and targeted therapeutic strategies.

Firstly, Candida albicans filaments' virulence factors, including secreted aspartyl proteases and phospholipases, are thought to be important. The underlying mechanisms of Candida and candidiasis and promising new directions in drug discovery and treatment.

Reviews all aspects of this common fungal pathogen and its impact on human health, from the basic biology of Candida albicans to the clinical management of candidiasis.; Reviews the latest basic and clinical research, focusing on findings in genome variability, host-pathogen Author: Richard A.

Calderone. Chronic biofilm infections are often accompanied by a chronic inflammatory response, leading to impaired healing and increased, irreversible damage to host tissues. Biofilm formation is a major virulence factor for Candida albicans and a challenge for treatment.

Most current antifungals have proved ineffective in eradicating infections attributed to by: REVIEW Targeting Candida albicans filamentation for antifungal drug development Taissa Vila a, #, Jesus A.

Romo, Christopher G. Pierceb, Stanton F. McHardyc, Stephen P. Savillea, and Jose L. Lopez-Ribota aDepartment of Biology and South Texas Center for Emerging Infectious Diseases, The University of Texas at San Antonio, San Antonio, TX, USA; bDepartment of Biology, University of the.

Incorporating amphotericin B into liposomes was reported to decrease amphotericin B toxicity without a concomitant loss of antifungal efficacy. We formulated an alternative emulsion-based delivery system for amphotericin B and compared it with Fungizone®, The maximal tolerated dose (MTD) in mice was 1mg of Fungizone/kg; however,the MTD was >9 Cited by: Chronic hyperplastic candidiasis (CHC) lesions will progress to dysplasia with some of these developing squamous cell carcinoma (SCC).

It is well known that diabetic patients are predisposed to candidiasis. Previously, we found that alloxan-induced diabetic rats spontaneously have mucosal hyperplasia with C. albicans infection and that those lesions progress to by: 5. F was effective (% 2-week survival, 10 mg/kg oral administration) in vivo in the treatment of invasive aspergillosis in a neutropenic murine model of infection.

DHODH is also present in mammalian cells, but F is a very poor inhibitor of the human form of the enzyme (half maximal inhibitory concentration >90 µM) giving a > Cited by:   Candida albicans morphogenesis and biofilm development.

(A) Transmission electron micrograph of a germinating yeast tube can be seen as a filament, which continues to elongate forming a hypha. (B) A schematic illustrating the stages of C. albicans biofilm nce: yeast cells adhere to a substrate forming a yeast basal layer. Cited by: Vaginal candidiasis, is caused by the overgrowth of a fungal species, Candida albicans, in the vaginal flora (Sobel, Faro et al.

). The symptoms of vulvovaginal candidiasis include pruritus (itching), soreness, change in vaginal discharge, and dyspareunia (Sobel ; Sobel ), and can disruptFile Size: KB.